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An estimated 0.8% of of 17.3 million clinic visits by women 18 yrs and older for urinary tract symptoms including neurogenic bladder & urinary retention between 2003-2013 were prescribed bethanechol (132,281 patients over 10 yrs) (Gaitonde et al, 2019)

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We observed a change in the fluorescence lifetime from 1.3 ns in an NADP + -saturated state to 2.3 ns in an NADPH-saturated state, which is exceptionally large for a fluorescent protein-based biosensor