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In one study performed in 2013 participants had to take 1000mg orally for 6 months to see a 30% increase in blood concentration
For those with mild deficiencies or whove stabilized with injections, this route can be convenient and effective
After overnight TEV digestion at 4 C, the GST elution was then loaded on a Superdex 75 Increased 10/300 GL column (Cytiva) preequilibrated with wash buffer
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