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A total of 5 g His-UBE2m and 5 g GST-PRMT1 were added into a standard 50-L reaction mixture containing 5 l S-adenosyl-l-methionine (SAM, Amersham Bioscience), 50 mM Tris-HCl (pH 8.0), 20 mM KCl, 5 mM DTT, and 4 mM EDTA

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Our experience with patients in metabolic therapy shows the biweekly 500mg schedule produces visible changes in truncal fat distribution within 68 weeks, while weekly 250mg protocols often show minimal body composition change even when total weight decreases