These symptoms are not specific to iron deficiency
Suppression of synaptic activity was achieved by changing cultured cells into serum-free base-medium (10% minimum essential media (MEM, Life Technologies) and 90% saltglucoseglycine (SGG) containing in mM: 114 NaCl, NaHCO3, 5.292 KCl, 1 MgCl2, 2 CaCl2, 10 HEPES, 1 Glycine, 30 Glucose, 0.5 sodium pyruvate, 0.1% Phenol Red) with 100 nM tetradotoxin (TTX, Tocris) on DIV9 for 24 h to inhibit neuronal action potential firing
For the chin, we lean towards the longer end, because the submental area is small and visible, and rushing the spacing makes it harder to see what one session has actually achieved
it's just spread across several days instead of landing in one shot