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The mice were then decapitated and the entire brain was removed and immediately submerged in ice-cold, carbogen-saturated (95% O 2 and 5% CO 2 ) aCSF (126 mM NaCl, 2.8 mM KCl, 1.2 mM MgCl 2 , 2.5 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 and 5 mM glucose)

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Sublingual administration allows the medication to be absorbed directly into the bloodstream, bypassing the digestive system

Fluorescence was measured using a fluorescence plate reader (Envison TM , PerkinElmer) at Ex/Em = 540/590 nm