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doi: 10.7759/cureus.36565, 116

3.2 Hydrothermal carbonization Hydrochar can be produced via hydrothermal carbonization (HTC) routes at a lower temperature (200250 C) than the pyrolysis carbonization pathway (300800 C) because hot water is available during the reaction

2.7 ELISA tests for inflammation marker assay Specific rat ELISA kits from SunLong Biotech Co., Ltd., Hangzhou City, Zhejiang, China (catalog numbers SL0722Ra, SL0411Ra, and SL0537Ra, respectively) were used to measure tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and NF-B in hepatic homogenates, which are key indicators of liver inflammation

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