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A 1 mL solution has 1000 mcg of Vitamin B12
Importantly, the first committed step of the pathway, catalyzed by glucose-6-phosphate dehydrogenase (G6PD), is inhibited by NADPH [57]
All participants were on metformin at the maximum tolerated dose according to clinical and laboratory parameters (mean dose = 1500 mg), and none were taking other antihyperglycemic drugs including insulin analogues
That means a 250mcg dose is only 2.5 units nearly impossible to measure accurately on an insulin syringe
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