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To verify DASPEI scores, fish from the same treatment groups were fixed and immunohistochemically processed with anti-parvalbumin (Millipore) to visualize hair cells (see Coffin et al., 3).These results indicate that Dihexa only modulates targets responsible for the acute phase of aminoglycoside ototoxicity
Protein carbonyls (marker of oxidative protein damage) were measured using the Protein Carbonyl Colorimetric assay (Cayman Chemical Company, Ann Arbor, MI, USA)
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