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As an initial application of the method, we generated a library of all possible single-residue substitutions to a 60-mer VAMP1 sequence and assessed the cleavage activities of wild-type BoNT/X and BoNT/F proteases on this library (Extended Data Fig
Long-term use may be required to maintain weight loss, which has cost and ongoing commitment implications
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The free amino acids that arrive in the bloodstream after digestion have no signaling specificity
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