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Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min, and the clarified cell lysate was directly used for biocatalytic reactions
37 MireaultM.RoseC
Choroid plexuses sampled from two-day-old animals were homogenized in lysis buffer (Cell lysis buffer , Cell Signaling Technology Inc.) supplemented with the protease inhibitor phenylmethanesulfonyle fluoride (1 mM, Sigma-Aldrich)
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