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The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord
ILC2s were identified as follows: live cells, CD45 + , lineage-negative (CD3, CD4, CD5, TCR, TCR, CD45R/B220, CD335, CD11c, CD11b, Gr-1, FcRI, and Ter119), ST2 + , and CD127 +
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A 1 mg dose draws 0.2 mL (20 units on a U-100 syringe), and a 0.5 mg dose draws 0.1 mL (10 units)
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