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There isn't enough safety data to support ipamorelin use during pregnancy or while nursing
Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
The resulting supernatants were aliquoted and used for Enzyme-Linked Immunosorbent Assay (ELISA) analyses
Neuron 96(4):897909 Scrocchi LA et al (1996) Glucose intolerance but normal satiety in mice with a null mutation in the glucagon-like peptide 1 receptor gene
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