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Working with a supplier that provides a batch-specific Certificate of Analysis remains the most direct way to remove that variable from a research protocol
Serum cytokine levels were measured using a custom-ordered Bio-Plex Pro rat Cytokine Assay kit (10014905, Bio-Rad, Hercules, CA, USA) to quantify five cytokines, based on the literature (86, 87): IL-1, IL-6, IL-10, TNF, and MCP-1 using the Bio-Plex 200 system (Bio-Rad) according to manufacturers instructions
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The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA
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