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at 37C in the presence of Zombie Red (1/1000) according to the manufacturers procedure (Ozyme) then trypsinized and fixed in PBS with formalin 3% (Sigma Aldrich)
In order for nutrients to reach the cell and work effectively, they must be absorbed and not digested
Polypodium leucotomos extract (PLE) is increasingly recognized for its potential in melasma management due to its powerful antioxidant and photoprotective effects
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