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after three washes, 50 l of HRP-conjugated goat anti-mouse IgG (Sigma) was added to each well and incubated at 37 C for 1 h
LCACs also affect Ca 2+ homeostasis between the mitochondria and the ER
Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling
It is also worth deciding in advance how many bottles a study will require, since ordering enough of a single lot for the full study reduces the chance of introducing batch-to-batch variability partway through a multi-week protocol
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