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Amplified products were visualized on a 1.5% agarose gel and purified using a kit (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel, Dren, Germany) according to manufacturers instructions, for subsequent Sanger sequencing
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More than half the time , they took those drugs without consulting their physician Follow Our Antibiotic Protocol A quick look at these numbers is another sobering reminder that people remain unaware about the real problems with antibiotic resistance, underscoring the findings of a 2019 report from Oregon State University that concluded 43 percent of antibiotics prescribed in America were completely unnecessary
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