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The UPR consists of three main signaling pathways, PERK, IRE-1, and ATF6., that normally bound by a chaperone called Grp78 and are inactive
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Pelleted cells were resuspended in Lysis A buffer (40 mM HEPES pH 7.5, 2 mM magnesium acetate, 200 mM NaCl, 10 mM DTT, 1 mM benzamidine, 10 g/mL leupeptin, 50 g/mL AEBSF), and stored at 20 C
Consequently, cationic CPPs, which are better suited for cellular uptake owing to their positive charge, are more effective for the cellular delivery of CAND
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