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After treating human hepatocytes with prednisolone, the active metabolite of prednisone, and CsA at concentrations ranging from 0.5 to 100 M for 96 h, the incubation medium was replaced with 100 M 4-trifluoromethyl-7-hydroxycoumarin and 100 M -estradiol, which are probe substrates of UGT1A9/2B7 and UGT1A1/1A3, respectively, for an additional incubation of 30 min
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According to previously assigned interval groups (7, 15, 30, 90, 180, and 360 days), the animals were sacrificed with an overdose of 3% isoflurane
Therefore, despite its hyperglycemic effects, glucagon triggers lipid catabolism, energy expenditure and reduces food intake
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