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After the incubation period (1 h), the solution was centrifuged for five minutes (10,000 rpm) at room temperature
using it to reduce abdominal fat outside that indication is an off-label application that should be discussed directly with a prescribing provider, and like the GLP-1 drugs above, it requires a prescription rather than being sold over the counter
The GSH level increased over the first 20 generations (two transfers), but subsequently declined and after 60 generations (six transfers) had returned close to that of the control, wild-type strain cultured in the same conditions (Figure S3A)
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