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The lentiviral particles for CBS OPT and L2HGDH OPT constructs were generated similarly with the packaging system as those for sgRNA but selected with puromycin at a final concentration of 1.5 g/ml
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Similarly, NEM or vinylpyridine is added to the glutathione recycling assay reaction mix, and the GSH content can be calculated by the subtraction of GSSG from total glutathione
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