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Sections were blocked with 5% goat serum (Cell Signaling), 1% BSA (Sigma) and 0.2% gelatin (Sigma) in PBS with 0.1% Triton-X for 1h at RT, and then incubated overnight with primary antibodies at 4 C
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Yes, you can take them on an empty stomach, but some people may experience mild nausea
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