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Pyridoxine facilitates GSH synthesis via the PKM2-Nrf2 pathway First, we performed an initial screen of 863 natural products purchased from TargetMol by intrinsic tryptophan differential scanning fluorimetry (nanoDSF) to explore molecules that may directly bind with PKM2
We introduced small quantities of anhydrous liquid pNPB or desiccated solid pNPPal substrates directly into the solvent-free liquid lipases prepared at just above the melting temperatures (Supplementary Information, Materials and Methods)
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