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The receptor-bound HPC4 resins were washed with a buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG, 0.006% (w/v) CHS, and 5 M semaglutide supplemented with 5 mM CaCl 2 before the bound material was eluted in a buffer supplemented with 10 mM EDTA