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Required Laboratory Materials IGF-1 LR3 lyophilized powder (1mg vial) Bacteriostatic water for injection or sterile water for injection Sterile low-protein-binding microcentrifuge tubes Calibrated micropipettes (10-1000L range) Sterile pipette tips with aerosol barrier Laminar flow hood or biological safety cabinet 70% isopropyl alcohol for surface sterilization Sterile gauze pads Laboratory-grade analytical balance Vortex mixer (optional) Refrigerated storage unit (-20C to +4C) Pre-Reconstitution Preparation Protocol Establish sterile working conditions within a laminar flow hood, ensuring all surfaces undergo thorough decontamination with 70% isopropyl alcohol
It also prevents the breakdown of collagen
Basrah J Vet Res 15(2):227245 Burillon C, Chiambaretta F, Pisella P-J (2018) Efficacy and safety of glycyrrhizin 2.5% eye drops in the treatment of moderate dry eye disease: results from a prospective, open-label pilot study
Mn2 + is also an excellent contrast agent for T1-weighted MRI
