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With proper sterile technique, you can continue sampling from the same vial for 3+ months the standard "discard after 28 days" disclaimer is an FDA bacteriostatic labeling requirement, not a reflection of peptide efficacy

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The mechanisms include reduction of excitotoxicity and oxidative stress, enhanced neurotrophic factor expression supporting neuronal survival, angiogenesis improving cerebral blood flow, and modulation of dopamine and serotonin systems

The mechanism researchers find most interesting is angiogenesis, the growth of new blood vessels