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It was proposed that an auxotrophic strain of Escherichia coli ( X 1776) could be used for all genetic modification practices, as the strain is unable to survive without the supplementation of DAP, and thus, cannot live outside of a laboratory environment
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Its potency can degrade by up to 10% in 96 hours, so it is recommended to use it within a week after opening
Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme
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