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Gene expression analysis Total RNA from cell lines was purified using QIAwave RNA Mini Kit (74536, QIAGEN) and total RNA from murine tissues was isolated by trituration with a motorized micro pestle, followed by column purification with QIAwave RNA Mini Kit (74536, QIAGEN)
Regenerative peptides: TB-500 (Thymosin beta-4): cell migration + actin | BPC-157: NO + EGF/VEGF + gastroprotection + neuroprotection | Stack BPC-157 + TB-500: documented preclinical synergy
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41 Many factors that normally stimulate ET-1 synthesis, (for example, thrombin and AT-II) also cause the release of vasodilators, such as prostacyclin (PGI 2 ) and/or NO, which oppose the vasoconstricting function of ET-1
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