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Typically, 20 ml of mid-log cell culture concentrated into 50 l of electrocompetent cell slurry yielded up to 10 6 transformants if 1 g of plasmid DNA is used per electroporation
Consistent with the above functional and biochemical findings, intracellular ROS levels assessed by DCFH-DA fluorescence staining showed that MAPKAPK2 overexpression markedly attenuated H2O2-induced ROS accumulation, reducing ROS levels by approximately 30%40% compared with the H 2 O 2 + vector group
Nodules often result in scarring on the face
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