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1), its chemical synthesis is complicated by the need for protection and deprotection of carboxyl and amino groups when introducing a sulfur atom at the C- 2 position of the imidazole ring

Notably, Compound 86 engaged only F392 and F424 and formed a single hydrogen bond with H267, whereas 8HP and DSR-141562 displayed additional interactions with H373, Q421, D370, and Y222, with 8HP exhibiting the highest affinity due to extra aromatic contacts (Figures 26AC)