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Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
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To further explore this citrate method Bally et al., studied the effect of proton gradient by replacing the intraliposomal solution to HEPES buffer pH (7.0) from 300 mm citrate buffer (pH 4.0), keeping the internal pH undisturbed
Consider the Injection Frequency : For frequent injections, using a syringe that provides ease of measurement and comfort is crucial
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