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IC50 was calculated according to cell viability using the nonlinear regression analyses in GraphPad Prism 10.0 software

Cagrilintide contains N-terminal acylation with a C20 fatty diacid (eicosanedioic acid) linked via a -glutamic acid spacer , along with an intramolecular disulfide bond between Cys3 and Cys8

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Alcohol consumption on DSIP nights is inadvisable, as alcohol already disrupts sleep architecture and combining it with DSIPs effects creates unpredictable outcomes