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Less Common and Potentially Serious Causes of a Chronic Sore Throat While less common, other causes of a chronic sore throat can include: Rarely, a serious condition like throat cancer can cause symptoms similar to a chronic sore throat

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(3) and (3) respectively transforming the recombinant expression vectors obtained in the step (2) into escherichia coli BL21(DE3) through a heat shock method, screening monoclonals through resistance, respectively selecting 8 clones from the two recombinant engineering bacteria, respectively naming the clones as A1-GLP-1-1 to A1-GLP-1-8 and A2-GLP-1-1 to A2-GLP-1-8, and storing the clones in glycerol