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The presented method demonstrates a sensitive quantitative method for a commercially available GLP-1 analog, semaglutide, in rat plasma

We thank Ms Colleen Pisaneschi for editing the manuscript and Sigma-Tau (Pomezia, Italy) for the gift of acetyl-L-carnitine

the most common side effects are gastrointestinal, including nausea, vomiting, and diarrhoea, consistent with the GLP-1 receptor agonist class

Other serious but rare side effects include pancreatitis (severe stomach pain, fever, nausea, vomiting), gallbladder problems (yellowing of skin or eyes, upper stomach pain), kidney injury (decreased urination, swelling), diabetic retinopathy (vision changes), severe allergic reactions (rash, swelling of face/throat, difficulty breathing), increased heart rate, and new or worsening depression or suicidal thoughts