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Overall, both X(GD_L)f and X(GD) exhibit more stringent substrate requirements than wild-type BoNT/X protease or X(PC)

Hypoglycemic medicines in the treatment of Alzheimers disease: Pathophysiological links between AD and glucose metabolism

Samples (50 L) were prepared at 100 M in sterile filtered 1X Phosphate Buffered Saline (PBS), pH 7.4, and centrifuged in a microfuge at 21,000g for 15 minutes before loading onto the FPLC

Fasting blood samples were collected, and plasma TC, triglyceride (TG), LDL-C, and high-density lipoprotein cholesterol (HDL-C) concentrations were measured using an automated biochemical analyzer (AU5400, Beckman Coulter, Brea, CA, United States)