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Cytokine profiling using immunology multiplex assay MCP-1/CCL2, IL-6, and TNF-alpha (TNF) were measured in fasted plasma collected at endpoint using the Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel in the Luminex MAGPIX system (EMD Millipore, Billerica, MA)

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The primary endpoints in the current study were the area under the serum icodec concentrationtime curve and the dose-normalized area under the plasma semaglutide concentrationtime curve from zero to last quantifiable observation after a single dose (AUC 0 t ,SD,Icodec and dose-normalized AUC 0 t ,SD,Semaglutide )

These follow-ups are also a time to talk through any changes in appetite, weight, or overall progress, and make sure your lifestyle modifications are still aligned with your goals