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The decision stems from the companys inability to reach alignment with the FDA on a feasible clinical trial design for expanding the drugs indication to non-dialysis #chronic #kidney #disease (#CKD) patients
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American Journal of Physiology-Endocrinology and Metabolism, 319(4), E659E666
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After protein quantification, loading buffer containing 10% SDS, 0.5% bromophenol blue in Tris-HCL (pH 6.8), and 10% -mercaptoethanol (M3148, Sigma) was added to each cell lysate and boiled at 95-100 C for 10 min
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