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Results and discussion Structure determination Human CTR, plus or minus individual RAMPs, and a stabilised form of Gs (dominant negative Gs (DNGs)) and G12, were co-expressed in Trichoplusia ni insect cells

Results tend to appear faster and more dramatically than topical application

Occludin monoclonal antibody (batch number 66378) and STAT3 polyclonal antibody (batch number 10253-2-AP) were obtained from Proteintech

Evans blue extravasation refers to the potential leakage of Evans blue dye from blood vessels into the surrounding tissues, which potentially indicates increased vascular permeability, possibly associated with inflammation