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Clean the injection site with an alcohol swab and allow to dry completely Pinch a fold of skin and subcutaneous tissue between thumb and forefinger Insert a 29 or 30 gauge insulin needle at a 45 degree angle into the pinched tissue Inject the solution slowly and steadily Withdraw the needle and apply gentle pressure if needed Rotate injection sites daily to prevent localized irritation Site Rotation Alternating between medial and lateral sides of the Achilles area prevents accumulation of scar tissue from repeated injections at the same spot

This process was repeated twice and concentrated in a rotary vacuum evaporator (Buchi R-300) to dryness

Aim the needle at the vial wall, not directly at the peptide powder

In addition, allergies, gastroesophageal reflux, constipation, dental caries, and other sources of pain are common