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Based on the amount of GSH in the lysate, we calculated the GSH concentration assuming an averaged HeLa/HT1080 cell volume of 4,000 m 3 (refs 15, 51, 52)
Protein available from xCT siRNA-1 showed a significant reduction in xCT expression (30.1%) relative to scramble control levels quantified by western blotting densitometry ( P =0.002) ( Online Supplementary Figure S7B )
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