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This was one of the first signals that the peptide wasn't just a wound-healing agent it had specific follicular activity
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
The Veterinary Clinics of North America: Small Animal Practice
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