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As described in previous studies (52, 53), the MRS of the ACC was performed using a short TE spin-echo full-intensity acquired localized single-voxel spectroscopy sequence (54) with the following parameters: TE = 8.5 ms, TR = 3,000 ms, 128 averages, and VOI dimensions = 30 20 20 mm 3
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Low-purity or degraded peptide produces poor results regardless of protocol
Repeated temperature cycling accelerates degradation noticeably more than steady storage at any single temperature inside the recommended bands minimise transitions between cold and ambient
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