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Loss of beta-cells leads to insulin insufficiency and hyperglycemia, with patients eventually requiring lifelong insulin therapy to maintain normal glycemic control
Under these conditions, reconstituted material is generally considered stable for up to 30 days
Subsequently, the cells were washed with PBS and stained by C11-BODIPY 581/591 (5 M) for 20 min
Even without these tools, consistent manual tracking enables data-driven decisions
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