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The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

Choline supports liver function and fat metabolism, helping to prevent the accumulation of fats in the liver and promoting their transport and utilization throughout the body B12 enhances metabolism, aids in the breakdown of fats and proteins for energy, and supports appetite regulation and energy levels L-Carnitine helps the body convert fat into energy, which can support weight loss efforts by enhancing fat metabolism and potentially reducing fat accumulation Leucine an amino acid, promotes fat oxidation, preserves muscle mass through muscle protein synthesis, and regulates appetite by influencing satiety hormones Vitamin C promotes fat oxidation, boosts energy production, supports metabolism, and may help regulate appetite Whens a good time to get a LipoLean Injection

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For those who are deficient, it may be necessary to supplement the diet with B12