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Michael Hammond, a recent graduate of the internal medicine residency program at Northwesterns McGaw Medical Center, led the analysis alongside colleagues at the Feinberg School of Medicine
However, combination studies are limited and no established guidelines exist for combining these peptides
Raster Image Correlation Spectroscopy (RICS) image capture and analysis INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto glass bottom MatTek dishes and labelled with SNAP-Surface 647 (S9136S, New England Biolabs) for 15 minutes at 37C in full media, washed and imaged in RMPI-1640 media without phenol red using a 100X oil objective on a Leica Stellaris 8 STED FALCON microscope from the FILM Facility at Imperial College London using confocal settings as previously described (81)
doi: 10.1089/thy.2016.0171 56 GuLYangJGongYMaYYanSHuangYet al
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