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Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions

Long-term exposure can damage bones and joints, causing pain and mobility issues

Phentermine is approved for short-term use only, typically a few weeks

The SUSTAIN and PIONEER trial programs showed that GLP-1 receptor agonists reduce albuminuria (protein in urine), a marker of kidney damage