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HepG2 cell line was purchased from American Type Culture collection and was maintained in Dulbecos Modified Eagles Medium with phenol red (DMEM, Lonza, Belgium) supplemented with 10% fetal bovine serum (Cambrex, Belgium), penicillin/streptomycin (Lonza, Belgium)
Presented for research literature review only Podolsky MI et al
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He recommends one of the following locations: Abdomen For subcutaneous injections, the skin a couple of inches left or right of the belly button is ideal since its easy to pinch, which pulls fatty tissue away from the musclea good idea since youre not aiming for the latter in this approach
J.BenceK
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