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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min
Wendel A
Sderberg C, Stl P, Askling J, Glaumann H, Lindberg G, Marmur J, et al
Endocrinology and Metabolism (Seoul)
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