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Invisible to HPLC and mass spec
The peptide portion contains three amino acidsglycine, histidine, and lysinewhile the histidine residue contributes strongly to copper coordination
Intensity ratios between the paramagnetic and diamagnetic states were computed for all assigned and non-overlapping 1 H, 15 N, and 1 H, 13 C HSQC cross-peaks, employing a correlation time of the electron-spin interaction vector of 4 ns 52,53
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