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To prepare the Transwell assay, murine melanoma cells (1 10 5 cells per insert) cultured in 200 l RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 m pores, Corning), while 800 l RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber
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2.5 Enzyme inhibition studies Trypsin, from bovine pancreas, was dissolved in Hanks Balanced Salt Solution (HBSS) at a concentration of 0.02 mg/mL
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