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HepG2 cell line was purchased from American Type Culture collection and was maintained in Dulbecos Modified Eagles Medium with phenol red (DMEM, Lonza, Belgium) supplemented with 10% fetal bovine serum (Cambrex, Belgium), penicillin/streptomycin (Lonza, Belgium)
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Several studies from Ole Madsen (University of Copenhagen) had shown that transplantation of glucagon producing tumours caused profound anorexia in animals
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